How to interpret ankle-brachial index (ABI) waveforms

Master how to interpret both audible and analog waveforms across stages of peripheral arterial disease.
Last update26th Feb 2021

During an automated ankle-brachial index (ABI) test, ABI ratios are calculated and included in the patient report. While waveforms are only audible when using Doppler during a manual ABI, an automated ABI machine’s Doppler probe also provides analog waveforms, which form an important component of the report.

Understanding how to interpret both the audible and analog waveforms of an automated ABI machine is especially important in circumstances where the ABI ratios are inaccurate and irrelevant. For example, some vessels are so calcified that they resist compression and falsely elevate the ABI ratio.

How to position the Doppler probe

Before we get into how to interpret audible and analog waveforms, let’s review how to position the Doppler probe when performing an ABI. Pulse sounds (e.g., audible waveforms) and analog waveforms are qualitative data produced by the Doppler probe. The sound is crucial, but it is not recorded. Only the analog waveforms are recorded, and their quality is dependent on the skill of the person using the Doppler probe.

It is easy to make a healthy vessel look diseased, but it is not easy to make a diseased vessel look healthy. In other words, audible and analog waveforms may overestimate the presence of disease if the technique is poor.

Good technique comes with practice and experience. Ideally, the pen is held at 45° (or less) to the vessel or skin, and is pointed towards the heart. The more perpendicular you are to the vessel, the more you will overestimate the severity of disease.

Figure 1. Good technique for a Doppler probe involves holding the pen at 45° or less to the vessel or skin and pointing towards the heart.

How to avoid waveform artifacts

Artifacts can appear on analog waveforms as multiple tiny peaks and pits. Artifacts are errors, not true peaks and pits, and they can make waveforms difficult to classify.

There are two reasons why artifacts may be introduced:

  1. The user has a shaky hand.
  2. There is venous flow interference.

A tip to avoid venous flow interference is to press gently on the vein with the probe. Since the vein has lower pressure, it will collapse. The venous flow will pause and allow for a clear arterial Doppler reading without artifacts.

Figure 2. An artifact on a waveform appears as multiple tiny peaks and pits, which can make the waveforms difficult to interpret and classify.

How to interpret audible and analog waveforms

In general, waveforms that consist of two peaks and one pit are ideal and considered normal. These are called triphasic waveforms.

As peripheral arterial disease (PAD) worsens from mild to severe, the waveforms change. First, they decrease in the number of peaks and pits, then they develop rounded upstrokes or humpback peaks (e.g., wide peaks without pits), and finally, all peaks and pits become absent.

There are five stages of waveform degradation that occur with worsening PAD:

  1. Biphasic (mild)
  2. Weak biphasic (moderate)
  3. High monophasic (moderate)
  4. Dampened monophasic (severe)
  5. Absent (critical)

First, let’s cover what triphasic waveforms look and sound like. Then, we will describe each stage of waveform degradation so you can master how to interpret both audible and analog waveforms across PAD stages.

Triphasic waveforms (normal)

The healthiest waveforms are called triphasic, meaning they have two peaks and one pit that are both audible and visible. A triphasic waveform indicates that a shift of direction in blood flow is occurring, which reflects normal vessel flexibility.

A triphasic waveform features a sharp incline to the tallest peak; the upstroke represents an acceleration of blood flow to a peak systole. The pit is early diastole, which is the reversal of flow. The second peak is late diastole, which is the forward flow. Essentially, triphasic waveforms represent blood flow that is normal at rest.

Figure 3. Normal, triphasic ankle-brachial index (ABI) waveforms feature a sharp upstroke to the tallest peak (blood flow acceleration during systole), a pit (reversal of flow during early diastole), and a second peak (forward flow during late diastole).

With audible triphasic waveforms, you’ll notice three distinct sounds that follow the pattern peak-pit-peak (e.g., one pit and two peaks).

Check out this short snippet from our Ultrasound Masterclass: Arteries of the Legs Course to listen to a normal, triphasic waveform:


Biphasic waveforms (mild)

Artery walls lose elasticity as they become diseased, which is reflected in a loss of humps in the ABI waveforms. Audible biphasic waveforms only have two sounds, and visually (e.g., on analog) have only one peak and one pit. Notably, biphasic waveforms still have a sharp upstroke to the tallest peak.

The loss of the second peak can sometimes be due to an asymptomatic loss of elasticity in the artery. This is due to wall calcification, which is worse with atherosclerosis and diabetes but can also develop naturally with age. Wall calcification can become severe at a younger age in patients with diabetes.

Patients with biphasic waveforms usually present with symptoms of intermittent claudication. However, biphasic waveforms can also be found in patients with mild to moderate arterial insufficiency. Correlating the waveforms with the ABI ratios helps decipher the cause of the biphasic waveforms. For example, if the ABI is 1.0 and the waveform is biphasic, there could be mild calcification in the vessels but no significant atherosclerosis. Biphasic waveforms obtained with an ABI of 0.7 indicate mild to moderate PAD.

Figure 4. Biphasic ankle-brachial index (ABI) waveforms caused by mild peripheral arterial disease (PAD) features a sharp upstroke, one peak, and one pit.

When listening to biphasic waveforms, you’ll notice only two distinct sounds. As long as the waveforms are multiphasic, biphasic, or triphasic (e.g., not monophasic), the situation is not an immediate surgical concern.

This short video from our Ultrasound Masterclass: Arteries of the Legs Course features what you can expect to hear when listening to biphasic waveforms:


Weak biphasic waveforms (moderate)

Waveforms with a sharp upstroke and shallow pits are called weak biphasic. They are found when there is moderate arterial insufficiency.

Weak biphasic waveforms are best analyzed using analog (not audible) waveforms. Similar to the biphasic waveforms, you can still expect to hear one peak and one pit. But, the difference with weak biphasic waveforms is that the upstroke (e.g., peak) is usually louder than the pit.

Figure 5. Weak biphasic ankle-brachial index (ABI) waveforms caused by moderate peripheral arterial disease (PAD) features a sharp upstroke, one peak, and shallow pits.

High monophasic waveforms (moderate)

When the waveforms have no pits but still have one peak with a sharp upstroke, they are called high monophasic. These waveforms also represent moderate arterial insufficiency.

Distinguishing between high monophasic and dampened monophasic waveforms can be difficult. It involves a subjective component which is dependent on the experience of the operator. Experienced operators can appreciate the sharp upstroke found with high monophasic—as opposed to the dampened waveforms that are seen with more severe forms of PAD.

Figure 6. High monophasic ankle-brachial index (ABI) waveforms caused by moderate peripheral arterial disease (PAD) features a sharp upstroke, one peak, and no pits.

When listening to high monophasic waveforms, you’ll only hear one sound. For an example of what high monophasic waveforms sound like, check out this video from our Ultrasound Masterclass: Arteries of the Legs Course:


Dampened monophasic waveforms (severe)

Dampened humpback monophasic waveforms indicate severe PAD. There is a slow-rising peak above the baseline and the upstroke is rounded—not sharp. Dampened monophasic waveforms can be seen with non-healing ischemic ulcers.

Figure 7. Dampened monophasic ankle-brachial index (ABI) waveforms caused by severe peripheral arterial disease (PAD) features a slow-rising peak above the baseline and a rounded upstroke.

When listening to dampened monophasic waveforms, you’ll hear only one peak and continuous flow throughout the cardiac cycles.

You can hear an example of dampened monophasic waveforms by checking out this video from our Ultrasound Masterclass: Arteries of the Legs Course:


Absent waveforms (critical)

Absent ABI waveforms are due to a lack of peripheral pulses. They are found in patients with rest pain and critical limb ischemia.

Figure 8. The absence of ankle-brachial index (ABI) waveforms is caused by a lack of peripheral pulses from critical peripheral arterial disease (PAD).

Become a great clinician with our video courses and workshops

Recommended reading

  • Aboyans, V, Criqui, MH, Abraham, P, et al. 2012. Measurement and interpretation of the ankle-brachial index: a scientific statement from the American Heart Association. Circulation126: 2890–2909. PMID: 23159553
  • Cervin, A, Wanhainen, A, and Björck, M. 2020. Popliteal aneurysms are common among men with screening detected abdominal aortic aneurysms, and prevalence correlates with the diameters of the common iliac arteries. Eur J Vasc Endovasc Surg59: 67–72. PMID: 31757587
  • Cleveland Clinic. 2021. Leg and foot ulcers. Cleveland Clinic
  • Cleveland Clinic. 2021. Marfan syndrome. Cleveland Clinic
  • Cleveland Clinic. 2021. Popliteal artery entrapment syndrome (PAES). Cleveland Clinic
  • Cleveland Clinic. 2021. Statin medications & heart disease. Cleveland Clinic
  • Collins, L and Seraj, S. 2010. Diagnosis and treatment of venous ulcers. Am Fam Physician81: 989–996. PMID: 20387775
  • Høyer, C, Sandermann, J, and Peterson, LJ. 2013. The toe-brachial index in the diagnosis of peripheral arterial disease. J Vasc Surg58: 231–238. PMID: 23688630
  • Jaoude, WA. 2010. Management of popliteal artery aneurysms. SUNY Downstate Department of Surgery
  • Johns Hopkins Medicine. 2021. Aneurysm. Johns Hopkins Medicine
  • Kassem, MM and Gonzalez, L. 2020. “Popliteal artery aneurysm”. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing.
  • Moxon, JV, Parr, A, Emeto, TI, et al. 2010. Diagnosis and monitoring of abdominal aortic aneurysm: current status and future prospects. Curr Probl Cardiol35: 512–548. PMID: 20932435
  • Richert, DL. 2016. Gundersen/Lutheran Ultrasound Department Policy and Procedure Manual. Gundersen Health System
  • Rivera, PA and Dattilo, JB. 2020. “Pseudoaneurysm”. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing.
  • Stanford Medicine 25. 2021. Measuring and understanding the ankle brachial index (ABI). Stanford Medicine 25
  • Teo, KK. 2019. Acute peripheral arterial occlusion. Merck Manuals Professional Edition
  • The Regents of the University of California. 2020. Diabetic foot ulcers. UCSF Department of Surgery
  • Zwiebel, WJ and Pellerito, JS. 2005. Introduction to Vascular Ultrasonography. 5th edition. Philadelphia: Elsevier Saunders. (Zwiebel and Pellerito 2005, 254–259)

About the author

Elizabeth Tenny, BS RVT RDCS
Elizabeth is a Registered Vascular Technologist in the Department of Vascular Surgery, Stanford Health Care, USA.
Author Profile